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Cellular distribution of rabbit IgA allotypes

W C Hanly, K L Knight

    Journal of Immunology (Baltimore, Md. : 1950)
    |August 1, 1975
    PubMed
    Summary
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    Rabbit plasma cells show allelic exclusion and subclass exclusion for alpha-chain subclasses f and g. Epithelial cells in Lieberkühn

    Area of Science:

    • Immunogenetics
    • Molecular Biology
    • Cell Biology

    Background:

    • Immunoglobulin heavy chains possess subclasses, with alpha-chain subclasses (f and g) being studied in rabbits.
    • Allelic exclusion is a phenomenon where only one immunoglobulin allele is expressed per cell.
    • Subclass exclusion, similar to allelic exclusion, may also occur at the subclass level.

    Purpose of the Study:

    • To investigate allelic and subclass exclusion of alpha-chain subclasses (f and g) in rabbit intestinal plasma cells and epithelial cells.
    • To determine if the expression of different genes within the heavy chain chromosomal region is coordinately regulated.

    Main Methods:

    • Intestinal tissue from heterozygous rabbits (at the f or g loci) was analyzed.
    • Fluorescently labeled antibodies, directed against allelic products of the f or g loci, were used.

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  • Rhodamine and fluorescein labels were employed to distinguish between maternal and paternal alleles.

    • Main Results:

    • Individual plasma cells exhibited both allelic and subclass exclusion, being stained by only one fluorescent reagent.
    • Epithelial cells of the Lieberkühn's glands did not show allelic exclusion.
    • In heterozygous rabbits, the ratio of maternal-type f cells to paternal-type f cells was equal to the ratio of maternal-type g cells to paternal-type g cells.

    Conclusions:

    • Plasma cells rigorously adhere to allelic and subclass exclusion for alpha-chain subclasses.
    • Epithelial cells of the Lieberkühn's glands do not exhibit allelic exclusion.
    • The data suggest coordinate control over the expression of genes within the heavy chain chromosomal region of a given allogroup.