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Related Experiment Videos

Glutaraldehyde and human T cell rosettes

J Evans, M A Smith, C M Steel

    Journal of Immunological Methods
    |July 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Glutaraldehyde alters sheep erythrocytes, increasing apparent rosette-forming cells by causing adherence to B lymphocytes. The sheep erythrocyte rosette test for T lymphocytes is more accurate without glutaraldehyde.

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    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • The rosette formation assay is used to identify lymphocyte populations.
    • Sheep erythrocytes are commonly used in rosette assays.

    Purpose of the Study:

    • To investigate the effect of glutaraldehyde on sheep erythrocyte rosette formation with human mononuclear cells.
    • To determine the accuracy of the rosette test for identifying T lymphocytes with and without glutaraldehyde treatment.

    Main Methods:

    • Human blood mononuclear cells and lymphoblastoid cell lines were incubated with sheep erythrocytes.
    • The effect of glutaraldehyde fixation of sheep erythrocytes and its addition before rosette scoring was examined.

    Main Results:

    • Glutaraldehyde increased the apparent proportion of rosette-forming cells.

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  • Glutaraldehyde-treated sheep erythrocytes adhered to B lymphocytes.
  • The rosette test with fresh sheep cells more accurately identified T lymphocytes than when glutaraldehyde was present.

    • Conclusions:

    • Glutaraldehyde fixation alters sheep erythrocytes, leading to non-specific binding with B lymphocytes.
    • For accurate T lymphocyte identification using the sheep erythrocyte rosette assay, glutaraldehyde should be avoided.