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Evidence of pestivirus RNA in human virus vaccines

R Harasawa1, T Tomiyama

  • 1Animal Center for Biomedical Research, Faculty of Medicine, University of Tokyo, Japan.

Journal of Clinical Microbiology
|June 1, 1994
PubMed

Insights

Pestivirus RNA was detected in some measles, mumps, and rubella vaccines. Sequence analysis confirmed that a cattle vaccine strain was not the source of this contamination in human vaccines.

Area of Science:

  • Virology
  • Vaccinology
  • Molecular Biology

Background:

  • Live virus vaccines are crucial for preventing infectious diseases.
  • Ensuring the purity and safety of vaccines is paramount for public health.
  • Pestiviruses are a genus of viruses that can affect livestock and potentially other species.

Purpose of the Study:

  • To investigate the presence of pestivirus RNA or pestiviruses in live virus vaccines.
  • To identify the potential source of pestivirus contamination in human vaccines.

Main Methods:

  • Reverse transcription PCR (RT-PCR) was employed to detect pestivirus RNA.
  • Nucleotide sequence analysis was performed on amplified PCR products.

Main Results:

  • Pestivirus RNA was detected in two measles-mumps-rubella (MMR) combined vaccines.
  • Pestivirus RNA was also found in two monovalent vaccines (one mumps, one rubella).
  • Sequence analysis ruled out a modified live bovine viral diarrhea virus vaccine strain as the source.

Conclusions:

  • Contamination with pestivirus RNA exists in some MMR and monovalent vaccines.
  • The identified pestivirus RNA in vaccines does not originate from the common modified live bovine viral diarrhea virus vaccine used in cattle.
  • Further investigation is needed to determine the origin and implications of pestivirus RNA in these vaccines.

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