Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Conditional regulatory elements of human immunodeficiency virus type 2 long terminal repeat

S K Arya1, J R Mohr

  • 1Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.

The Journal of General Virology
|September 1, 1994
PubMed
Summary

Researchers discovered new regulatory elements in the human immunodeficiency virus type 2 (HIV-2) long terminal repeat (LTR) that control viral gene expression. These elements function differently across cell types, impacting HIV-2 replication.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Donor demographics and factors affecting corneal utilisation in Eye Bank of North India.

International ophthalmology·2021
Same author

Usability of donor corneas harvested from the deceased having septicaemia or malignancy.

QJM : monthly journal of the Association of Physicians·2019
Same author

Effect of Variable Oxidation States of Vanadium on the Structural, Optical, and Dielectric Properties of B<sub>2</sub>O<sub>3</sub>-Li<sub>2</sub>O-ZnO-V<sub>2</sub>O<sub>5</sub> Glasses.

The journal of physical chemistry. B·2016
Same author

Correlation of progression of diabetic retinopathy with the alterations in retrobulbar circulation.

Nepalese journal of ophthalmology : a biannual peer-reviewed academic journal of the Nepal Ophthalmic Society : NEPJOPH·2013
Same author

Safety and efficacy of low-molecular-weight heparins in prophylaxis of deep vein thrombosis in postoperative/ICU patients: A comparative study.

Journal of natural science, biology, and medicine·2013
Same author

Retinal nerve fiber layer thickness in Indian eyes with optical coherence tomography.

Nepalese journal of ophthalmology : a biannual peer-reviewed academic journal of the Nepal Ophthalmic Society : NEPJOPH·2012

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • The human immunodeficiency virus type 2 (HIV-2) long terminal repeat (LTR) is crucial for viral gene expression and replication.
  • Understanding the regulatory mechanisms of HIV-2 LTR is essential for developing antiviral strategies.

Purpose of the Study:

  • To identify and characterize novel cis-acting regulatory elements within the HIV-2 LTR.
  • To investigate the cell type-specific activity of these elements and their interaction with viral proteins like Tat.

Main Methods:

  • Mutational analysis of the HIV-2 LTR U3 region.
  • Reporter gene assays in various human cell lines (CEM, Jurkat, U937, HeLa).
  • Assessment of Tat-mediated trans-activation and T cell activation responses.

Related Experiment Videos

Main Results:

  • A novel positive and a negative regulatory element were identified in the upstream U3 region of the HIV-2 LTR.
  • These elements exhibited cell type-specific activity, with varying effects in lymphocytic, monocytic, and epithelioid cells.
  • The negative regulatory element's effect was abrogated by HIV-2 Tat, indicating its role in Tat-mediated trans-activation.
  • T cell activation signals were also mapped to the upstream U3 region.
  • The impact of these regulatory elements was dependent on the basal LTR activity, which itself was cell type-specific.

Conclusions:

  • The HIV-2 LTR contains cell type-specific regulatory elements in the U3 region that influence viral gene expression.
  • These findings highlight the importance of cell-specific transcriptional factors in regulating HIV-2 replication.
  • The identified elements and their interactions with Tat may contribute to the cell type-specific restriction of HIV-2 replication in vivo.