Type-2 astrocytes have much greater susceptibility to heat stress than type-1 astrocytes

The present investigations were undertaken to examine the susceptibility of type-2 astrocytes to elevated temperature. Type-2 astrocytes are much more easily injured by temperature elevation than type-1 astrocytes. This may be related to cellular redox potential. Type-1 astrocytes have a greater cytosolic NAD redox potential (i.e., higher NADH:NAD levels) than type-2 astrocytes as evidenced by a 9-fold higher ratio of lactate to pyruvate released into the medium by type-1 astrocytes than type-2 astrocytes. Heat stress causes the induction of hsp-72 in both type-2 and type-1 astrocytes; however, hsp-72 protein expression is retained for a longer period of time by the type-2 astrocyte. A possible basis for the greater sensitivity of type-2 astrocytes to stress may be a poorer ability to scavenge free radicals. This differential sensitivity of one neural cell type relative to another to elevated temperature may be of significance in understanding the effects of hyperthermia on the developing brain.