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Sequences and evolutionary analysis of mouse 5S rDNAs

H Suzuki1, K Moriwaki, S Sakurai

  • 1Division of Molecular Genetics, Jikei University School of Medicine, Tokyo, Japan.

Molecular Biology and Evolution
|July 1, 1994
PubMed
Summary

Mouse 5S ribosomal RNA (rRNA) spacer regions were amplified and sequenced. These rapidly evolving regions show reduced heterogeneity within genomes and can aid in phylogenetic analysis of closely related species.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Evolutionary Biology

Background:

  • 5S ribosomal RNA (rRNA) genes are tandemly repeated in the mouse genome.
  • Spacer regions between these genes exhibit length heterogeneity.

Purpose of the Study:

  • To analyze the sequence and evolutionary dynamics of mouse 5S rRNA spacer regions.
  • To assess the utility of these regions for phylogenetic studies.

Main Methods:

  • Polymerase chain reaction (PCR) amplification of 5S rRNA spacer regions from four mouse strains.
  • Cloning and sequencing of amplified DNA fragments.
  • Sequence divergence analysis.

Main Results:

  • Amplified fragments were approximately 1.6 kb and GC-rich.
  • Sequences contained repetitive motifs like (TG)n and (ATCC)n, contributing to length variation.
  • Intragenomic sequence divergence averaged 0.26%, while interspecific divergence reached 1.73%.

Conclusions:

  • Mouse 5S rRNA spacer regions evolve rapidly, with mechanisms reducing intragenomic heterogeneity.
  • These spacer regions are valuable markers for phylogenetic analysis of closely related species.

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