Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A common core structure for U3 small nucleolar RNAs

T Hartshorne1, N Agabian

  • 1Intercampus Program in Molecular Parasitology, School of Pharmacy, University of California, San Francisco 94143-1204.

Nucleic Acids Research
|August 25, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultrasound screening for abdominal aortic aneurysm in high-risk women.

The British journal of surgery·2021
Same author

Pharmacogenetic allele nomenclature: International workgroup recommendations for test result reporting.

Clinical pharmacology and therapeutics·2015
Same author

Antigenic variation in African trypanosomes: DNA rearrangements program immune evasion.

Immunology today·2014
Same author

Comments regarding 'Assessment of the accuracy of AortaScan for detection of abdominal aortic aneurysm (AAA)'.

European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·2012
Same author

Reprinted article "Subintimal angioplasty of femoropopliteal artery occlusions: the long-term results".

European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·2011
Same author

Extracranial and transcranial ultrasound assessment in patients with suspected positional 'vertebrobasilar ischaemia'.

European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·2009

This study models the Trypanosoma brucei U3 small nucleolar RNA (snRNA), revealing unique structural features. Key differences in its 3' domain suggest these structures aren't essential for conserved U3 snRNA functions.

Area of Science:

  • Molecular Biology
  • RNA Biology
  • Parasitology

Background:

  • U3 small nucleolar RNA (snRNA) plays a crucial role in the early processing of primary ribosomal RNA (rRNA) transcripts.
  • Understanding the structure of U3 snRNA is vital for elucidating its function in gene expression and cellular processes.

Purpose of the Study:

  • To determine the secondary structure model of the unusually small U3 snRNA in Trypanosoma brucei.
  • To compare conserved and divergent features of U3 snRNAs across different species.
  • To investigate the impact of protein interactions on U3 snRNA structure.

Main Methods:

  • Chemical modification and enzymatic cleavage of U3 snRNA in both deproteinized and ribonucleoprotein (RNP) forms.
  • Comparative sequence alignment of U3 snRNAs from diverse species (vertebrate, plant, fungal, protozoan).

Related Experiment Videos

  • Structural probing to analyze specific sequence configurations.

    • Main Results:

    • A secondary structure model for Trypanosoma brucei U3 snRNA was deduced, featuring a flexible 5' stem loop and a long single-stranded region.
    • Comparative analysis revealed conserved single-stranded sequences in the 3' domain across species.
    • Structural probing indicated that protein interactions alter the GAU region, box B, and box C sequences in U3 RNP.
    • Trypanosomal U3 snRNAs notably lack stem loops II and III found in other species' U3 snRNAs.

    Conclusions:

    • The deduced secondary structure model for T. brucei U3 snRNA presents a variation on previously proposed models.
    • Conserved single-stranded regions in the 3' domain suggest their importance in U3 snRNA function.
    • The absence of stem loops II and III in trypanosomal U3 snRNAs implies these structures are not universally required for conserved U3 snRNA functions.