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Related Experiment Videos

A 92-kDa human immunostimulatory protein

E Fontan1, E Briend, H Saklani-Jusforgues

  • 1Unité d'Immunophysiologie Cellulaire, Institut Pasteur, Paris, France.

Proceedings of the National Academy of Sciences of the United States of America
|August 30, 1994
PubMed
Summary
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A human urinary glycoprotein (HGP.92) demonstrated potent anti-Listeria activity and inhibited tumor cell growth in mice. Its biological functions were linked to its glycan moiety, suggesting potential therapeutic applications.

Area of Science:

  • Biochemistry
  • Immunology
  • Glycobiology

Background:

  • A novel human urinary glycoprotein, HGP.92 (92 kDa), was purified.
  • This glycoprotein exhibits significant biological activities.

Purpose of the Study:

  • To investigate the protective effects of HGP.92 against Listeria monocytogenes infection.
  • To explore the anti-tumor properties of HGP.92.
  • To determine the role of the glycan moiety in HGP.92's biological activities.

Main Methods:

  • Intravenous administration of HGP.92 to mice.
  • In vitro incubation of inflammatory macrophages with HGP.92.
  • N-Glycanase treatment to assess the role of oligosaccharides.

Main Results:

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  • HGP.92 (250 µg/kg) conferred complete protection against lethal Listeria monocytogenes infection in mice.
  • Protection was observed in severe combined immunodeficient (SCID) mice, indicating a non-lymphocyte-mediated mechanism.
  • HGP.92 (6 nM) inhibited Lewis carcinoma cell growth in vitro when incubated with inflammatory macrophages.
  • Both activities were abolished by N-Glycanase treatment, highlighting the importance of the oligosaccharide component.

Conclusions:

  • The biological activities of HGP.92, including anti-Listeria and anti-tumor effects, are dependent on its glycan moiety.
  • HGP.92 represents a promising candidate for further investigation in infectious disease and cancer therapy.