Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A 92-kDa human immunostimulatory protein

E Fontan1, E Briend, H Saklani-Jusforgues

  • 1Unité d'Immunophysiologie Cellulaire, Institut Pasteur, Paris, France.

Proceedings of the National Academy of Sciences of the United States of America
|August 30, 1994
PubMed
Summary

A human urinary glycoprotein (HGP.92) demonstrated potent anti-Listeria activity and inhibited tumor cell growth in mice. Its biological functions were linked to its glycan moiety, suggesting potential therapeutic applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Poor stimulus discriminability as a common neuropsychological deficit between ADHD and reading ability in young children: a moderated mediation model.

Psychological medicine·2016
Same author

Mechanisms underpinning inattention and hyperactivity: neurocognitive support for ADHD dimensionality.

Psychological medicine·2014
Same author

[Anaesthetic management of a parturient with a severe factor VII deficiency: was activated factor VII really necessary?].

Annales francaises d'anesthesie et de reanimation·2014
Same author

[Silent sinus syndrome. Clinical case].

Archivos de la Sociedad Espanola de Oftalmologia·2013
Same author

Aortic thrombus and multiple embolisms during a Mycoplasma pneumoniae infection.

Infection·2013
Same author

Specificity of basic information processing and inhibitory control in attention deficit hyperactivity disorder.

Psychological medicine·2013

Area of Science:

  • Biochemistry
  • Immunology
  • Glycobiology

Background:

  • A novel human urinary glycoprotein, HGP.92 (92 kDa), was purified.
  • This glycoprotein exhibits significant biological activities.

Purpose of the Study:

  • To investigate the protective effects of HGP.92 against Listeria monocytogenes infection.
  • To explore the anti-tumor properties of HGP.92.
  • To determine the role of the glycan moiety in HGP.92's biological activities.

Main Methods:

  • Intravenous administration of HGP.92 to mice.
  • In vitro incubation of inflammatory macrophages with HGP.92.
  • N-Glycanase treatment to assess the role of oligosaccharides.

Main Results:

Related Experiment Videos

  • HGP.92 (250 µg/kg) conferred complete protection against lethal Listeria monocytogenes infection in mice.
  • Protection was observed in severe combined immunodeficient (SCID) mice, indicating a non-lymphocyte-mediated mechanism.
  • HGP.92 (6 nM) inhibited Lewis carcinoma cell growth in vitro when incubated with inflammatory macrophages.
  • Both activities were abolished by N-Glycanase treatment, highlighting the importance of the oligosaccharide component.

Conclusions:

  • The biological activities of HGP.92, including anti-Listeria and anti-tumor effects, are dependent on its glycan moiety.
  • HGP.92 represents a promising candidate for further investigation in infectious disease and cancer therapy.