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Related Experiment Videos

Vacuolar H(+)-pumping ATPase variable transport coupling ratio controlled by pH

J M Davies1, I Hunt, D Sanders

  • 1Biology Department, University of York, United Kingdom.

Proceedings of the National Academy of Sciences of the United States of America
|August 30, 1994
PubMed
Summary

This study reveals that the proton-transporting efficiency of vacuolar-type proton ATPases (V-ATPases) in red beets varies with pH. This pH-dependent regulation suggests V-ATPases control organelle acidification.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Plant Physiology

Background:

  • The eukaryote endomembrane system utilizes vacuolar-type proton ATPases (V-ATPases) for organelle acidification.
  • V-ATPase activity is vital for numerous physiological processes.
  • Regulatory mechanisms controlling V-ATPase activity remain incompletely understood.

Purpose of the Study:

  • To determine the thermodynamic efficiency (n) of proton transport per ATP hydrolyzed for the red beet V-ATPase.
  • To investigate the influence of cytoplasmic and lumenal pH on V-ATPase activity.
  • To elucidate potential regulatory roles of V-ATPases in controlling organelle pH.

Main Methods:

  • Thermodynamic determination of the H+/ATP ratio (n).
  • Utilized patch clamp technique.

Related Experiment Videos

  • Investigated V-ATPase from red beet vacuoles.

    • Main Results:

    • The H+/ATP ratio (n) for the red beet V-ATPase ranged from 1.75 to 3.28.
    • The value of n was strictly dependent on both cytoplasmic and lumenal pH.
    • A V-ATPase efficiency (n) below 2 was observed, explaining high H+ accumulation in plant vacuoles.

    Conclusions:

    • V-ATPases are regulated by cytoplasmic and lumenal pH.
    • V-ATPases may actively control cytoplasmic and lumenal pH.
    • The pH-dependent efficiency of V-ATPases is crucial for achieving low vacuolar pH in plants.