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Related Experiment Videos

Histone interactions in solution and susceptibility to denaturation

D E Roark, T E Geoghegan, G H Keller

    Biochemistry
    |July 13, 1976
    PubMed
    Summary
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    Histone interactions are sensitive to purification methods. Nondenatured histones dissociate into monomers at low ionic strength, revealing structural insights into histone complexes like the (H4)2(H3)2 tetramer.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Structural Biology

    Background:

    • Histone interactions are crucial for DNA packaging and gene regulation.
    • Purification methods can potentially alter histone conformation and interactions.

    Purpose of the Study:

    • To assess histone susceptibility to denaturation from various treatments.
    • To investigate histone complex behavior in solution under varying ionic strengths.

    Main Methods:

    • Optical rotatory dispersion (ORD) measurements.
    • Sedimentation-velocity analytical ultracentrifugation.
    • Histone solutions exposed to reference solvent and denaturing agents (acid, urea, guanidine).

    Main Results:

    • Certain acid conditions, urea, and guanidine solutions caused irreversible histone denaturation.

    Related Experiment Videos

  • Nondenatured histone complexes (H4)(H3) dimer and (H4)2(H3)2 tetramer dissociated to monomers at low ionic strength.
  • Sedimentation-velocity data supported a compact, semispherical model for the (H4)2(H3)2 tetramer with extended amino-termini.
  • Histone H2a and H2b formed a mixed dimer, while H2a also self-associated into higher-order structures.
  • Histone H1 showed weak dimerization.

    • Conclusions:

    • Histone integrity and interactions are highly dependent on solution conditions and prior treatments.
    • Low ionic strength promotes dissociation of specific histone complexes into monomers.
    • The study provides a structural model for the (H4)2(H3)2 tetramer and details the oligomerization behavior of H2a, H2b, and H1.