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Related Experiment Videos

In vitro-lined endothelium: initial integrity and ultrastructural events

P Zilla1, P Preiss, P Groscurth

  • 1Department of Cardio-Thoracic Surgery, University of Cape Town Medical School, South Africa.

Surgery
|September 1, 1994
PubMed
Summary

In vitro-endothelialized vascular grafts maintained a confluent endothelium in baboons, showing initial cell loss but remaining non-thrombogenic. This suggests a promising approach for prosthetic vascular grafts.

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Area of Science:

  • Biomaterials Science
  • Vascular Surgery
  • Tissue Engineering

Background:

  • Assessing the early performance of in vitro-endothelialized prosthetic vascular grafts in a nonhuman primate model.
  • Investigating the fate of endothelial cells on artificial vascular surfaces.

Purpose of the Study:

  • To evaluate the patency and endothelial integrity of in vitro-endothelialized polytetrafluoroethylene (PTFE) grafts.
  • To compare the performance of endothelialized grafts versus control grafts in vivo.

Main Methods:

  • Utilized 17 male chacma baboons, implanting both control and endothelial cell (EC)-lined PTFE grafts.
  • Precoated grafts with fibrin glue and lined with autologous ECs, culturing them to confluence before implantation.
  • Monitored grafts at 9 days and 4 weeks post-implantation.

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Main Results:

  • Endothelial-lined grafts maintained confluent endothelium without fibrin deposits at 9 days, despite initial cell density reduction.
  • Intercellular gaps and inflammatory cells were observed initially, with recovery of high cell density and mature morphology by 4 weeks.
  • Control grafts exhibited significant fibrin deposits and protein coverage.

Conclusions:

  • In vitro-endothelialized vascular prostheses demonstrate sustained endothelial confluency post-implantation.
  • Despite moderate initial endothelial cell loss, these grafts exhibit non-thrombogenic properties.
  • The findings support the potential of in vitro endothelialization for improving vascular graft performance.