Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spontaneous injection in microcolumn separations

H A Fishman1, N M Amudi, T T Lee

  • 1Department of Chemistry, Stanford University, California 94305.

Analytical Chemistry
|July 15, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of laying parity and sex ratio on reproduction performance and biochemical parameters of White Roman geese kept in an environmentally controlled house.

British poultry science·2024
Same author

Effect of the age and sex on growth performance and feather quality of 13 to 25-weeks-old White Roman geese.

Poultry science·2023
Same author

Effects of fermented soybean meal with Bacillus velezensis, Lactobacillus spp. or their combination on broiler performance, gut antioxidant activity and microflora.

Animal bioscience·2022
Same author

Exploring the Effectiveness of the Introduction of Automated Physiological Monitoring Devices.

Studies in health technology and informatics·2021
Same author

Effects of dietary supplementation with Taiwanese tea byproducts and probiotics on growth performance, lipid metabolism, and the immune response in red feather native chickens.

Animal bioscience·2020
Same author

Laetiporus sulphureus-fermented wheat bran enhanced the broiler growth performance by improving the intestinal microflora and inflammation status.

Poultry science·2020
Same journal

Heterojunction-Enhanced Interfacial Evanescent-Tunable Fiber Optic Probe for Amplification-free CRISPR/Cas12a-Based Rapid and Ultrasensitive Detection of MPXV.

Analytical chemistry·2026
Same journal

Tunable Charge Transfer in Europium Metal-Organic Frameworks for Ratiometric Sensing of a Sarin Simulant.

Analytical chemistry·2026
Same journal

A β-Cyclodextrin/Ag<sub>2</sub>O@MWCNT-Based Stochastic Platform for the Simultaneous Molecular Enantiorecognition and Enantioanalysis of Twelve Amino Acids in Biological Matrices.

Analytical chemistry·2026
Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026
Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026
Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026
See all related articles

Spontaneous injection in microcolumn separations is mainly caused by interfacial pressure differences. Optimizing capillary inlet design and controlling timing significantly improves quantitative precision and separation efficiency for ultramicrosampling.

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Fluid Dynamics

Background:

  • Spontaneous injection in microcolumn separations impacts quantitative precision and separation efficiency.
  • Ultramicrosampling techniques require precise control over injection volumes.

Purpose of the Study:

  • To characterize the phenomenon of spontaneous injection in microcolumn separations.
  • To identify the primary cause of spontaneous injection.
  • To develop strategies for improving quantitative precision and separation efficiency.

Main Methods:

  • Combined fluorescence imaging, video microscopy, and capillary electrophoresis measurements.
  • Fluid dynamics modeling with simplifying assumptions.
  • Experimental manipulation of capillary inlet geometry and buffer evaporation.

Related Experiment Videos

Main Results:

  • Spontaneous injection is primarily caused by an interfacial pressure difference at the capillary inlet.
  • Etching the capillary inlet or using thin-walled capillaries reduced extraneous injection up to 12-fold.
  • Controlled timing and buffer management significantly influenced injection length.

Conclusions:

  • Understanding interfacial pressure is key to controlling spontaneous injection.
  • Capillary inlet modifications and precise timing are effective strategies for enhancing ultramicrosampling.
  • This research improves the reliability and efficiency of microseparation techniques.