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GPI-specific phospholipase D as an apolipoprotein

M A Deeg1

  • 1Department of Medicine, University of Washington, Seattle 98195.

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas
|February 1, 1994
PubMed
Summary

Glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) primarily binds to apolipoprotein AI in human plasma. This enzyme is associated with a small, distinct particle, not the main high-density lipoprotein (HDL) fraction.

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Area of Science:

  • Biochemistry
  • Lipid Metabolism
  • Enzymology

Background:

  • Glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) has been linked to high-density lipoproteins (HDL) in bovine serum.
  • Understanding GPI-PLD's distribution in human plasma is crucial for elucidating its biological roles.

Purpose of the Study:

  • To determine the distribution of GPI-PLD across different lipoprotein fractions in human plasma.
  • To characterize the specific lipoproteins that contain GPI-PLD activity.

Main Methods:

  • Utilized dextran sulfate and immunoaffinity chromatography for lipoprotein fractionation based on apolipoproteins (ApoB, ApoAI/AII, ApoAI).
  • Employed gel filtration and various gel electrophoresis techniques for detailed particle characterization.
  • Assayed GPI-PLD activity within isolated lipoprotein fractions.

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Main Results:

  • Approximately 75% of GPI-PLD activity was associated with apolipoprotein AI-containing lipoproteins (Lp AI).
  • Minimal activity was found in apolipoprotein AI/AII (Lp AI/AII) or lipoprotein-deficient plasma; none was detected in apolipoprotein B (Lp B) containing lipoproteins.
  • GPI-PLD was found in a small particle (approx. 140 kDa) distinct from bulk HDL, associated with apolipoprotein AI.

Conclusions:

  • The majority of human plasma GPI-PLD is specifically associated with apolipoprotein AI.
  • GPI-PLD resides in a distinct, small particle fraction containing apolipoprotein AI, separate from major HDL particles.