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Updated: Jul 16, 2026

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
Simplified high-performance liquid chromatographic method for propranolol and five metabolites in liver perfusate,
1College of Pharmacy, University of Saskatchewan, Saskatoon, Canada.
Abstract:
A new method has been developed for the analysis of propranolol plus five major metabolites in perfusate from isolated liver preparations, and also in rat serum and dog plasma. The regional isomers of hydroxypropranolol are clearly separated within a total run time of less than 15 min. The basic and neutral metabolites are extracted and analysed together, while the acidic metabolites are extracted in a second step. The new assay is more simple and time efficient than previously published methods.
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