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Effect of protein kinase C activators on the uptake of GABA by rat brain synaptosomes
A Cupello1, B Gasparetto, P Mainardi
1Centro di Neurofisiologia, C.N.R., Genova, Italy.
The International Journal of Neuroscience
|March 1, 1993
Abstract:
Synaptosomes were prepared from rat brain by a discontinuous Ficoll gradient method and used for studying the uptake of labelled GABA. Two GABA uptake components were evidenced, a high (Km = 3.13 microM) and a low (Km = 92.4 microM) affinity one. Preincubation of synaptosomes with two different activators of protein kinase C, phorbol 12, 13-diacetate (PDAc) and oleyl-acetyl glycerol (OAG), resulted in a change of GABA uptake. In particular, the low affinity component increased its Vmax by 58-74%, with no change in the Km. No statistically significant modification was detected for the high affinity component.