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Multiple adenovirus serotypes use alpha v integrins for infection
P Mathias1, T Wickham, M Moore
1Department of Immunology, Scripps Research Institute, La Jolla, California 92037.
Journal of Virology
|October 1, 1994
Summary
Human adenoviruses use a specific Arg-Gly-Asp (RGD) sequence in their penton base proteins to infect host cells. This RGD domain interacts with alpha v integrins, highlighting their crucial role in adenovirus entry.
Area of Science:
- Virology
- Molecular Biology
- Cellular Biology
Background:
- Adenoviruses are common human pathogens responsible for various infections.
- Viral entry mechanisms are crucial for understanding infection and developing antiviral strategies.
- Integrins are cell surface receptors involved in cell adhesion and signaling.
Purpose of the Study:
- To investigate the role of the penton base protein's integrin-binding domain in human adenovirus infection.
- To identify the specific host cell receptors involved in adenovirus entry.
Main Methods:
- Homology PCR was used to obtain nucleotide sequences encoding penton base integrin-binding domains.
- Bioinformatic analysis was performed to predict protein structure and identify conserved sequences.
Main Results:
- The penton base proteins of several human adenoviruses contain a conserved Arg-Gly-Asp (RGD) sequence.
- This RGD sequence is predicted to be located at the apex of two extended alpha helices.
- The penton base RGD domain facilitates efficient infection by multiple adenovirus serotypes through interaction with alpha v integrins.
Conclusions:
- Alpha v integrins are central to the entry of human adenoviruses into host cells.
- The penton base RGD domain is a key determinant of adenovirus host cell tropism and infectivity.