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A conserved sequence in tRNA and rRNA promoters of Lactococcus lactis
1Department of Genetics, Chr. Hansen's Laboratorium, Hørsholm, Denmark.
Biochimica Et Biophysica Acta
|September 13, 1994
Abstract:
A tRNA operon (trnA) from Lactococcus lactis consisting of seven tRNA genes and a 5S rRNA gene was cloned and sequenced. Promoter-fusion of the trnA promoter to a promoter-less beta-galactosidase gene of Leuconostoc mesenteroides resulted in high levels of beta-galactosidase activity in L. lactis. Searching for sequences with similarity to the sequence of the promoter region revealed a consensus sequence of promoters preceeding rRNA operons and tRNA operons from Lactococcus species including a not previously described conserved sequence (AGTT).