Related Experiment Videos
Instability of human TATA-binding protein CAG triplet repeats during amplification by PCR
F C Holstege1, P C van der Vliet, H T Timmers
1Laboratory for Physiological Chemistry, Utrecht University, The Netherlands.
Biochimica Et Biophysica Acta
|September 13, 1994
Abstract:
Polymerase chain reaction (PCR) of a TATA-binding protein cDNA that contains CAG triplet repeats results in heterogeneous products. This is caused by a variable loss in the number of CAG triplets. Sequence analysis of PCR products suggests that instability increases with repeat length.