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Characterization of the 5'-regulatory region of rat Reg I gene
H Miyashita1, H Yonekura, M Unno
1Department of Biochemistry, Tohoku University School of Medicine, Miyagi, Japan.
Biochimica Et Biophysica Acta
|September 13, 1994
Abstract:
We report here the characterization of the 5'-regulatory region of rat Reg I gene encoding a growth stimulating factor for pancreatic beta-cells. Transient expression assays of the 5'-flanking region/luciferase fusion gene in AR4-2J cells showed that the -304/-237 region contained positive cis-acting elements. Gel shift assays using AR4-2J and rat pancreas nuclear extracts showed the formation of a specific complex with the -256/-237 oligonucleotide.