Ectopic expression of a viral adenine methyltransferase gene in tobacco

Plant genomes contain both methylated adenine and cytosine residues although the roles of these methylations are not well understood. A chlorella virus adenine methyltransferase gene under the control of cauliflower mosaic virus 35S promoter in a binary plant transformation vector was expressed both in transgenic tobacco plants and transformed tobacco calli. The transgenic plants as well as transformed calli produced functional adenine methyltransferase enzyme, but the level of expression was higher in tobacco calli. A transgenic tobacco cell line that expressed the methyltransferase enzyme and carried an Arabidopsis cab3 gene containing a single target site for the adenine methyltransferase enzyme showed that the adenine residue was not methylated. HPLC analysis of genomic DNA from transgenic calli also showed no detectable levels of methylated adenine residues.