Transvitreal chorioretinal biopsy

Area of Science:

• Ophthalmology
• Surgical Innovation
• Histopathology

Background:

• Chorioretinal biopsy is crucial for diagnosing posterior eye diseases.
• Existing biopsy techniques may pose risks to ocular structures.
• A need exists for a minimally invasive and effective biopsy method.

Purpose of the Study:

• To describe a novel technique for transvitreal chorioretinal biopsy.
• To evaluate the feasibility and safety of the developed method.
• To assess the histological quality of biopsy specimens.

Main Methods:

• Development of a stereotaxic intraocular micromanipulator and biopsy instrument.
• Performance of transvitreal biopsy by trephinating a 1.5-mm posterior eye wall specimen.
• Achieving hemostasis and adhesion via mechanical compression.
• Sealing the defect with a silicone explant and promoting fibrovascular ingrowth.

Main Results:

• Successful retrieval of 1.5-mm posterior eye wall specimens.
• Effective hemostasis and chorioretinal adhesion achieved.
• Histological features preserved, with noted edema and minor retinal pigment epithelium detachment.

Conclusions:

• The developed transvitreal chorioretinal biopsy technique is feasible and safe.
• This method allows for adequate histological assessment of posterior eye wall tissues.
• The technique offers a promising approach for ocular disease diagnosis.