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Purification of microtubule proteins from Xenopus egg extracts: identification of a 230K MAP4-like protein
1Cell Biology Program, European Molecular Biology Laboratory, Heidelberg, Germany.
Cell Motility and the Cytoskeleton
|January 1, 1994
Abstract:
We describe the purification of microtubule proteins from Xenopus egg extracts by temperature-dependent assembly and disassembly in the presence of dimethyl sulfoxide and identify a number of presumptive microtubule-associated proteins (MAPs). One of these proteins has a molecular weight of 230 kDa and is immunologically related to HeLa MAP4. We show that this MAP is heat stable and phosphorylated, and that it promotes elongation of microtubules from axonemes.