Related Experiment Videos
Isolation and sequence of the Candida albicans FAS1 gene
1Department of Microbiology and Immunology, Georgetown University, Washington, DC 20007.
Abstract:
The gene (FAS1) encoding the beta-subunit of fatty-acid synthase (FAS) of Candida albicans has been isolated and analyzed. The gene was isolated on the basis of homology to the Saccharomyces cerevisiae FAS1 gene. Sequence analysis showed that the gene contained an intron-free open reading frame of 6111 bp encoding a protein of 2037 amino acids (aa) (227 916 Da). C. albicans FAS1 and its product exhibit a high degree of overall sequence relatedness to their counterparts in S. cerevisiae, with identities of 68 and 63% at the nucleotide (nt) and aa level, respectively. In addition, the beta-subunits of both organisms contain the catalytic domains in an identical sequential order. Northern blots demonstrated that the gene encodes a single mRNA of approx. 6.1 kb, and that changes in transcript levels are not a prerequisite of the yeast-to-hyphal transition. Southern blot analysis of C. albicans chromosomes separated by pulsed-field gel electrophoresis showed that FAS1 resides on chromosome 5.