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The chitinase encoding Tn7-based chiA gene endows Pseudomonas fluorescens with the capacity to control plant
1Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Gene
|September 15, 1994
Abstract:
A transposon system based on Tn7 carrying the chiA gene (encoding chitinase) of Serratia marcescens was established. The transposon was introduced by conjugation into Pseudomonas fluorescens as a stable integrate expressing and secreting the active chitinase enzyme. The resulting strain was shown to control the plant pathogen Rhizoctonia solani in soil.