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Related Experiment Videos

Morphine stimulates superoxide formation by glomerular mesangial cells

P C Singhal1, M Pamarthi, R Shah

  • 1Department of Medicine, Long Island Jewish Medical Center, New Hyde Park, New York 11042.

Inflammation
|June 1, 1994
PubMed
Summary

Morphine, a heroin metabolite, increases superoxide production in kidney mesangial cells. This suggests morphine may contribute to kidney damage in opiate abuse patients.

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Area of Science:

  • Nephrology
  • Toxicology
  • Cell Biology

Background:

  • Focal glomerulosclerosis is a common kidney lesion in heroin users.
  • Heroin's metabolite, morphine, is investigated for direct effects on kidney cells.

Purpose of the Study:

  • To determine if morphine directly stimulates superoxide production in glomerular mesangial cells.
  • To explore the role of opiate receptors in morphine-induced superoxide formation.

Main Methods:

  • Cultured rat glomerular mesangial cells were treated with varying concentrations of morphine.
  • Superoxide production was measured using spectrophotometric assays.
  • The effect of naloxone, an opiate antagonist, was assessed.

Main Results:

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  • Morphine significantly increased superoxide production in mesangial cells in a dose-dependent manner (P < 0.001).
  • Naloxone partially reversed the morphine-induced increase in superoxide production.
  • Control cells produced 401 +/- 21 nM/mg protein/h, while morphine-treated cells produced 610 +/- 41 nM/mg protein/h.

Conclusions:

  • Morphine enhances superoxide formation by glomerular mesangial cells.
  • This effect appears to be mediated via opiate receptors.
  • Morphine may play a role in the mesangial injury observed in opiate abuse.