Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Extended-spectrum beta-lactamase in Proteus mirabilis

S Mariotte1, P Nordmann, M H Nicolas

  • 1Laboratoire de Microbiologie, Hôpital Ambroise Paré, Faculté de Médicine, Paris-Ouest, Boulogne, France.

The Journal of Antimicrobial Chemotherapy
|May 1, 1994
PubMed
Summary

Five intensive care unit patients were infected by Proteus mirabilis resistant to extended-spectrum cephalosporins. A novel plasmid, pAP1, mediated this resistance, differing from previously identified TEM-3 beta-lactamase plasmids.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting the outer membrane porin OprF restores carbapenem efficacy during experimental sepsis caused by NDM-1-producing Pseudomonas aeruginosa.

Scientific reports·2026
Same author

Efficacy of imipenem combined with dimercaptosuccinic acid in a murine sepsis model using Pseudomonas aeruginosa.

Scientific reports·2025
Same author

Outbreak of Pseudomonas aeruginosa producing VIM carbapenemase in an intensive care unit and its termination by implementation of waterless patient care.

Critical care (London, England)·2021
Same author

Efficacy of dual carbapenem treatment in a murine sepsis model of infection due to carbapenemase-producing Acinetobacter baumannii.

The Journal of antimicrobial chemotherapy·2020
Same author

Evaluation of the performance of rapid tests for screening carriers of acquired ESBL-producing Enterobacterales and their impact on turnaround time.

The Journal of hospital infection·2020
Same author

Dimercaptosuccinic acid in combination with carbapenems against isogenic strains of Escherichia coli producing or not producing a metallo-β-lactamase in vitro and in murine peritonitis.

The Journal of antimicrobial chemotherapy·2020

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Molecular Biology

Background:

  • Extended-spectrum cephalosporins are crucial antibiotics.
  • Antibiotic resistance in intensive care units (ICUs) poses a significant threat.
  • Proteus mirabilis is an opportunistic pathogen often associated with healthcare-associated infections.

Purpose of the Study:

  • To investigate the emergence of extended-spectrum cephalosporin resistance in Proteus mirabilis within an ICU.
  • To characterize the genetic basis of this antibiotic resistance.
  • To determine the epidemiological relatedness of the resistant isolates.

Main Methods:

  • Phenotypic characterization of Proteus mirabilis isolates, including antibiotic susceptibility testing and metabolic profiling.

Related Experiment Videos

  • Molecular analysis to identify beta-lactamase production, including isoelectric focusing (pI) and gene hybridization.
  • Plasmid analysis, including conjugation experiments, size determination, restriction enzyme digestion (EcoRI), and incompatibility grouping.
  • Antimicrobial resistance gene identification (e.g., AAC(6')).

    • Main Results:

    • Five identical isolates of Proteus mirabilis resistant to extended-spectrum cephalosporins were identified in an ICU over one month.
    • All isolates produced a novel plasmid-mediated extended-spectrum beta-lactamase (ESBL) similar to TEM-3/CTX-1.
    • The resistance determinants were located on a unique 47 kb plasmid (pAP1), which also conferred aminoglycoside (AAC(6')) and sulphonamide resistance.
    • Plasmid pAP1 exhibited a low transfer frequency and did not belong to known Enterobacteriaceae incompatibility groups, distinguishing it from other TEM-3 plasmids.

    Conclusions:

    • A distinct plasmid, pAP1, is responsible for the spread of extended-spectrum cephalosporin resistance in Proteus mirabilis within this ICU.
    • The unique characteristics of pAP1 suggest a potentially novel mechanism of resistance dissemination.
    • This finding highlights the need for vigilant surveillance of emerging antibiotic resistance mechanisms in critical care settings.