Evidence for other binding component(s) than retinoid receptors specifically interacting with a retinoic acid...

A nuclear component interacting with a retinoic acid response element (RARE-beta) derived from the all-trans-retinoic acid receptor beta (RAR beta) gene promoter was detected in the rat liver by gel-shift assay. Competition experiments using the competitors of the other retinoid response elements and non-specific sequences upon the binding of this hepatic component (HBC) to the RARE-beta revealed that the binding of the HBC to the RARE-beta was competed only by the self competitor, but not the other elements, suggesting that the HBC is specific for the RARE-beta. Moreover, although the specific monoclonal antibodies for RAR alpha, RAR beta, and RAR gamma reacted and shifted up the DNA complexes of endogenous as well as recombinant RARs, the DNA complex with HBC was not subjected to the immunoreaction with the antibodies. Thus, newly identified HBC, which is distinct from the characterized retinoid receptors, may modulate retinoid signaling through its direct binding upon the RARE-beta.