Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Acute promyelocytic leukemia: morphological aspects

G L Castoldi1, V Liso, G Specchia

  • 1Institute of Hematology, University of Ferrara, Italy.

Leukemia
|September 1, 1994
PubMed
Summary

Acute promyelocytic leukemia (APL) is a distinct subtype of AML with maturation, presenting with varied cytological features. Diagnosis relies on cytochemistry, cytogenetics, and microscopy to differentiate subtypes and related conditions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-World Efficacy and Safety of Gemtuzumab Ozogamycin (GO) and 3 + 7 regimen in fit newly diagnosed Acute Myeloid Leukemia (AML) patients. A Retrospective multicenter study of "Rete Ematologica Pugliese" (REP).

Leukemia research reports·2025
Same author

Treatment outcomes in sinonasal aspergillosis in dogs in the United Kingdom: 436 cases (2011-2021).

The Journal of small animal practice·2025
Same author

Choice of Frontline Tyrosine-Kinase Inhibitor and Early Events in Very Elderly Patients With Chronic Myeloid Leukemia in Chronic Phase: A "Campus CML" Study.

European journal of haematology·2024
Same author

Diagnostic findings in sinonasal aspergillosis in dogs in the United Kingdom: 475 cases (2011-2021).

The Journal of small animal practice·2024
Same author

Daratumumab plus bortezomib or daratumumab plus lenalidomide as salvage therapy for patients with myeloma: initial follow-up of an Italian multicentre retrospective clinical experience by 'Rete Ematologica Pugliese'.

Annals of hematology·2022
Same author

Monitoring minimal residual disease by ddPCR in acute lymphoblastic leukemia associated with the FGFR1 gene rearrangement.

International journal of laboratory hematology·2018

Area of Science:

  • Hematology
  • Oncology
  • Pathology

Background:

  • Acute promyelocytic leukemia (APL) is a specific subtype of acute myeloid leukemia (AML) with maturation.
  • APL constitutes 5-10% of all French-American-British (FAB) variants.
  • APL exhibits diverse cytological presentations, including hypergranular, microgranular, and hyperbasophilic forms.

Purpose of the Study:

  • To describe the distinct cytological and cytochemical characteristics of APL subtypes.
  • To highlight the differential diagnostic challenges and methods for APL.
  • To discuss a recently described entity associated with basophilic differentiation.

Main Methods:

  • Morphological examination of bone marrow aspirates.
  • Cytochemical staining (myeloperoxidase, Sudan black B, chloroacetate esterase, non-specific esterase).
  • Cytogenetic investigations and electron microscopy for differential diagnosis.

Main Results:

  • Hypergranular APL shows abundant granules; microgranular APL has fine or absent granules and bilobed nuclei; hyperbasophilic APL displays high N/C ratio and basophilic cytoplasm.
  • Typical APL (M3) shows strong positivity for myeloperoxidase, Sudan black B, and chloroacetate esterase; M3v shows weaker reactivity.
  • Cytochemical heterogeneity and a distinct basophilic variant of APL have been observed.

Conclusions:

  • APL subtypes have characteristic morphological and cytochemical profiles.
  • Accurate diagnosis requires a combination of morphology, cytochemistry, and potentially cytogenetics/electron microscopy.
  • Distinguishing APL from other myeloid neoplasms with basophilia is crucial for appropriate management.

Related Experiment Videos