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Role of H-2 and non-H-2-related genes in mouse susceptibility to Chlamydia psittaci
D Buzoni-Gatel1, F Bernard, M Pla
1UFR Pharmacie, Laboratoire d'Immunologie Parasitaire, Tours, France.
Abstract:
The genetic control of mouse susceptibility to Chlamydia psittaci was investigated after intravenous inoculation of 2 x 10(5) plaque-forming units (pfu). Splenic counts 6 days after inoculation gave the level of susceptibility. Results from inoculation of mice from H-2 congenic strains with three different genetic backgrounds (B10, BALB and C3H) suggested that both the non-H-2 genes and the genes of H-2 complex or those closely associated with it, were responsible for the observed differences in the innate capacity of various inbred lines of mice to control bacterial load in their infected organs following a challenge with C. psittaci.
Insights
Mouse genetic factors influence Chlamydia psittaci infection. Both H-2 complex genes and other non-H-2 genes control bacterial load in mice, impacting disease susceptibility.
Area of Science:
- Immunology
- Genetics
- Microbiology
Background:
- Mouse models are crucial for understanding Chlamydia psittaci pathogenesis.
- Genetic background significantly influences host-pathogen interactions.
Purpose of the Study:
- To investigate the genetic control of mouse susceptibility to Chlamydia psittaci.
- To identify specific genetic elements (H-2 and non-H-2 genes) influencing innate immunity against C. psittaci.
Main Methods:
- Intravenous inoculation of inbred mice with Chlamydia psittaci (2 x 10^5 pfu).
- Assessment of splenic bacterial load 6 days post-inoculation to determine susceptibility.
- Utilizing H-2 congenic mouse strains on diverse genetic backgrounds (B10, BALB, C3H).
Main Results:
- Significant differences in splenic bacterial counts were observed among different inbred mouse lines.
- Both H-2 complex-linked genes and unlinked non-H-2 genes contribute to controlling C. psittaci infection.
- Genetic background dictates the innate capacity to manage bacterial load.
Conclusions:
- Mouse inbred lines exhibit varying susceptibility to Chlamydia psittaci due to genetic factors.
- Both MHC (H-2) and non-MHC genes play a role in the immune response to C. psittaci.
- Understanding these genetic determinants is key for developing effective control strategies.