Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Murine oligodendroglial cells express nerve growth factor

S Byravan1, L M Foster, T Phan

  • 1Mental Retardation Research Center, University of California School of Medicine, Los Angeles 90024.

Proceedings of the National Academy of Sciences of the United States of America
|September 13, 1994
PubMed
Summary

Oligodendroglia, including precursor cells, express nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF). This suggests these cells actively support neurite extension beyond their role in myelination.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Search for Light Pseudoscalar Bosons, Pair-Produced in Higgs Boson Decays in the Four-Electron Final State in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

Observation of Suppressed Charged-Particle Production in Ultrarelativistic Oxygen-Oxygen Collisions.

Physical review letters·2026
Same author

Measurement of D^{0} Meson Photoproduction in Ultraperipheral Heavy Ion Collisions.

Physical review letters·2026
Same author

Observation of tWZ Production at the CMS Experiment.

Physical review letters·2026
Same author

First Exclusive Reconstruction of the B^{*+}, B^{*0}, and B_{s}^{*0} Mesons and Precise Measurement of Their Masses.

Physical review letters·2026
Same author

Simultaneous Probe of the Charm and Bottom Quark Yukawa Couplings Using tt[over ¯]H Events.

Physical review letters·2026

Area of Science:

  • Neuroscience
  • Cell Biology
  • Developmental Biology

Background:

  • Oligodendrocytes are glial cells crucial for myelin sheath formation in the central nervous system.
  • Neurotrophic factors like NGF and BDNF are vital for neuronal survival, growth, and differentiation.
  • The specific roles of oligodendroglia in supporting neuronal processes beyond myelination are not fully understood.

Purpose of the Study:

  • To investigate the expression of neurotrophic factors, specifically nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF), by oligodendroglial cells.
  • To determine if oligodendrocytes and their precursors produce NGF and BDNF and if these factors influence neuronal cells.

Main Methods:

  • Utilized an immortalized oligodendroglial cell line (N19) and primary mouse oligodendroglial cultures.

Related Experiment Videos

  • Employed polymerase chain reaction (PCR) and Northern blot analysis to detect NGF and BDNF mRNA expression.
  • Applied in situ hybridization histochemistry and immunocytochemistry to localize NGF mRNA and protein within specific oligodendrocyte populations (GC+ and A2B5+ cells).

    • Main Results:

    • The N19 oligodendroglial cell line expressed mRNAs for both NGF and BDNF.
    • Primary cultured oligodendrocytes (9-day cultures) were found to express NGF mRNA.
    • NGF mRNA and protein were localized within galactocerebroside (GC)-positive mature oligodendrocytes and A2B5-positive oligodendrocyte progenitors.

    Conclusions:

    • Oligodendroglia and their precursor cells are capable of expressing nerve growth factor (NGF).
    • These findings indicate that the oligodendrocyte lineage may actively contribute to neurite extension and regeneration.
    • This suggests a broader functional role for oligodendroglia in neural tissue, extending beyond their primary myelination function.