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Related Experiment Videos

BCL2 translocation frequency rises with age in humans

Y Liu1, A M Hernandez, D Shibata

  • 1Department of Molecular Pharmacology and Toxicology, School of Pharmacy, University of Southern California, Los Angeles 90033.

Proceedings of the National Academy of Sciences of the United States of America
|September 13, 1994
PubMed
Summary

The oncogenic t(14;18) chromosomal translocation, linked to B-cell leukemia/lymphoma-2 (BCL2), is common in healthy individuals. These BCL2 translocation clones persist and increase with age, suggesting a role in lymphoma development.

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Area of Science:

  • Hematology
  • Genetics
  • Oncology

Background:

  • The t(14;18) chromosomal translocation at the BCL2 locus is implicated in B-cell malignancies.
  • Understanding the prevalence and behavior of this translocation in healthy individuals is crucial for lymphoma research.

Purpose of the Study:

  • To determine the background frequency of the t(14;18) chromosomal translocation in peripheral blood lymphocytes (PBLs) and spleen tissues.
  • To investigate the relationship between translocation frequency, age, and B-cell populations.
  • To assess the persistence of t(14;18)-bearing clones.

Main Methods:

  • Nested PCR assay used to detect t(14;18) translocations.
  • Analysis of PBLs from 53 living individuals and spleen tissues from 31 autopsies.

Related Experiment Videos

  • Quantification of translocation frequency per million cells.

    • Main Results:

    • The t(14;18) translocation was detected in 55% of PBLs and 35% of spleens.
    • Translocation frequency ranged from less than 1 to 853 per million cells and was associated with B lymphocytes.
    • Translocation frequency significantly increased with age in both PBLs and spleens, with older individuals (≥61 years) showing substantially higher frequencies than younger individuals (≤20 years).
    • Specific t(14;18)-bearing clones persisted for at least 5 months in two individuals.

    Conclusions:

    • Clones harboring the oncogenic t(14;18) translocation are common in healthy humans.
    • These translocation-harboring clones are long-lived and increase in frequency with advancing age.
    • A multihit model of lymphomagenesis, involving initial t(14;18) translocation followed by antigen stimulation, is proposed.