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Replicon typing of 71 multiresistant Serratia marcescens strains
C Llanes1, M Couturier, L Asfeld
1Laboratoire de Bactériologie, CHU/Hôpital Jean Minjoz, Besançon, France.
Research in Microbiology
|January 1, 1994
Summary
Replicon typing identified plasmids in 52% of multiresistant Serratia marcescens strains. This method classified plasmids into specific replicon (rep) groups, often correlating with incompatibility (Inc) groups.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Plasmids are crucial in bacterial adaptation and antibiotic resistance.
- Replicon typing is a method to identify and classify plasmids based on their maintenance genes.
- Plasmid classification into replicon (rep) groups often correlates with incompatibility (Inc) groups.
Purpose of the Study:
- To classify plasmids from multiresistant Serratia marcescens strains using rep probes.
- To investigate the correlation between rep groups and known Inc groups.
- To determine the prevalence of different rep groups in the studied strains.
Main Methods:
- Utilized 19 rep probes derived from reference plasmid replicons of known Inc groups.
- Applied DNA hybridization techniques to 71 multiresistant Serratia marcescens strains.
- Performed electrophoretic analysis of plasmid DNA.
Main Results:
- Plasmids were identified and classified in 52% of the strains into FIB, FIC, FIIA, HI2, L/M, N, B/O, P, W, Y, and Com9 rep groups.
- Most plasmid preparations (79%) hybridized with a single rep probe.
- 21% of preparations showed hybridization with two different probes, indicating either multiple plasmids or a single multireplicon plasmid.
Conclusions:
- Replicon typing is effective for classifying plasmids in Serratia marcescens.
- The identified rep groups provide insights into plasmid diversity and evolution.
- Double hybridization highlights the complexity of plasmid content in multiresistant strains.