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Updated: Sep 4, 2026

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo
Published on: September 18, 2014
Synergistic activation of transcription by bacteriophage lambda cI protein and E. coli cAMP receptor protein
J K Joung1, D M Koepp, A Hochschild
1Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.
Abstract:
Two heterologous prokaryotic activators, the bacteriophage lambda cI protein (lambda cI) and the Escherichia coli cyclic AMP receptor protein (CRP), were shown to activate transcription synergistically from an artificial promoter bearing binding sites for both proteins. The synergy depends on a functional activation (positive control) surface on each activator. These results imply that both proteins interact directly with RNA polymerase and thus suggest a precise mechanism for transcriptional synergy: the interaction of two activators with two distinct surfaces of RNA polymerase.
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