Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Epidermal growth factor receptor activation in developing rat kidney

A V Cybulsky1, P R Goodyer, A J McTavish

  • 1Department of Medicine, Royal Victoria Hospital, Montreal, Quebec, Canada.

The American Journal of Physiology
|September 1, 1994
PubMed
Summary

During late gestation, fetal rat kidneys show increased epidermal growth factor receptor (EGFR) activity, crucial for perinatal kidney development. This heightened EGFR tyrosine kinase activity returns to basal levels after birth.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biallelic DICER1 mutations occur in Wilms tumours.

The Journal of pathology·2013
Same author

Rapid loss of renal parenchyma after acute obstruction.

Pediatric nephrology (Berlin, Germany)·2002
Same author

Complement C5b-9 induces cyclooxygenase-2 gene transcription in glomerular epithelial cells.

American journal of physiology. Renal physiology·2001
Same author

Cytosolic phospholipase A2-alpha associates with plasma membrane, endoplasmic reticulum and nuclear membrane in glomerular epithelial cells.

The Biochemical journal·2000
Same author

PAX2 suppresses apoptosis in renal collecting duct cells.

The American journal of pathology·2000
Same author

Complement C5b-9-mediated arachidonic acid metabolism in glomerular epithelial cells : role of cyclooxygenase-1 and -2.

The American journal of pathology·2000

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Renal Physiology

Background:

  • Epidermal growth factor (EGF) binding and receptor (EGFR) levels change significantly during rat kidney development.
  • EGFR activation is a key signaling pathway in cell growth and differentiation.

Purpose of the Study:

  • To investigate the changes in EGFR protein expression and tyrosine kinase activity in late-gestational rat kidneys.
  • To determine the role of EGFR activation in perinatal renal development.

Main Methods:

  • Measurement of EGF binding and EGFR protein levels in fetal and adult rat kidney membranes.
  • Assessment of endogenous tyrosine phosphorylation of EGFR and its substrates in kidney membranes.
  • Identification of phosphoproteins using molecular weight markers.

Related Experiment Videos

Main Results:

  • EGF binding and EGFR protein content increase in late-gestational rat kidneys compared to adults.
  • EGFR tyrosine kinase activity is significantly elevated (30-fold) in fetal kidney membranes.
  • The EGFR substrate phospholipase C-gamma 1 is tyrosine phosphorylated in fetal but not adult kidneys.

Conclusions:

  • EGFR tyrosine kinase is highly active in late-gestational rat kidneys, suggesting its critical role in perinatal renal development.
  • Increased EGFR signaling likely mediates fetal kidney cell proliferation and maturation.
  • EGFR activation decreases to adult levels postnatally.