Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dexamethasone inhibits basophil migration

M Yamaguchi1, K Hirai, K Nakajima

  • 1Department of Medicine and Physical Therapy, University of Tokyo School of Medicine, Japan.

Allergy
|May 1, 1994
PubMed
Summary

Dexamethasone directly inhibits basophil migration, a key step in allergic inflammation. This glucocorticoid action contributes to reducing basophil accumulation during late-phase reactions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Changes in platelet kinetics after a partial splenic arterial embolization in cirrhotic patients with hypersplenism.

Hepatology (Baltimore, Md.)·1995
Same author

Identification of a chromosomally encoded kanamycin acetylase in Porphyromonas gingivalis.

FEMS microbiology letters·1995
Same author

Pharmacological study of stem-cell-factor-induced mast cell histamine release with kinase inhibitors.

Biochemical and biophysical research communications·1995
Same author

Chemotactic agonists induce cytokine generation in eosinophils.

Journal of immunology (Baltimore, Md. : 1950)·1995
Same author

Epstein-Barr virus activity in patients on chronic hemodialysis.

Nephron·1995
Same author

Induction of eosinophil cytokine generation by chemoattractants.

International archives of allergy and immunology·1995

Area of Science:

  • Immunology
  • Pharmacology

Background:

  • Glucocorticoids reduce basophil accumulation in allergic late-phase reactions (LPR).
  • Basophil migration from circulation is crucial for recruitment during LPR.

Purpose of the Study:

  • To investigate if dexamethasone (DEX) directly inhibits basophil migration.
  • To assess DEX's effect on migration induced by C5a, IL-3, and IL-8.

Main Methods:

  • Purified human basophils were preincubated with varying DEX concentrations.
  • Basophil migration was induced using chemoattractants C5a, IL-3, and IL-8.
  • Cell viability was assessed using the trypan-blue exclusion test.

Main Results:

  • DEX demonstrated dose-dependent inhibition of basophil migration, with significant effects at 1 nM.

Related Experiment Videos

  • DEX inhibited migration induced by all tested chemoattractants similarly.
  • Inhibitory effects were rapid and not reversed by washing, without inducing toxicity.

    • Conclusions:

    • Dexamethasone directly inhibits basophil migration.
    • This direct action contributes to the in vivo suppression of basophil accumulation during LPR.