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Insulin-responsive regions of the rat fatty acid synthase gene promoter
1Institute of Food Research, Genetics and Microbiology Department, Colney, Norwich, United Kingdom.
Biochemical and Biophysical Research Communications
|September 15, 1994
Abstract:
Here we show that insulin may play a role in the diet-induced regulation of the rat fatty acid synthase (FAS; EC 2.3.1.85). Transient transfection of human and rat hepatoma cell lines with successively deleted FAS/CAT promoter fusion plasmids was used to determine the effect of insulin on FAS promoter activity. Our results indicate the existence of cis-acting insulin-responsive elements in the FAS promoter; the position of one of these is coincident with the position of a previously determined diet-induced DNAse I hypersensitive site (HSi-1) at approximately -500 bp relative to the transcription start site of FAS mRNA.