Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease of memory T helper cells (CD4+ CD45R0+) in hairy cell leukemia

F A van der Horst1, A van der Marel, G J den Ottolander

  • 1Department of Hematology, University Hospital Leiden, The Netherlands.

Leukemia
|January 1, 1993
PubMed
Summary

Hairy cell leukemia (HCL) patients show reduced CD4+ T cells expressing CD45R0, indicating impaired T-cell function and potential susceptibility to infections. This contrasts with other B-cell leukemias.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Midostaurin: a magic bullet that blocks mast cell expansion and activation.

Annals of oncology : official journal of the European Society for Medical Oncology·2017
Same author

Characterization of a New Human B Cell Line (Bonna-12) with Trisomy 9 and Trisomy 12 Chromosomal Abnormality.

Leukemia & lymphoma·2016
Same author

Hairy Cell Leukemia and Its T Cell Interactions.

Leukemia & lymphoma·2016
Same author

The impact of a male or female thrombotic family history on contraceptive counseling: a cohort study.

Journal of thrombosis and haemostasis : JTH·2016
Same author

KIT mutation analysis in mast cell neoplasms: recommendations of the European Competence Network on Mastocytosis.

Leukemia·2015
Same author

Newly diagnosed and relapsed mantle cell lymphoma: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up.

Annals of oncology : official journal of the European Society for Medical Oncology·2014

Area of Science:

  • Immunology
  • Hematology
  • Oncology

Background:

  • Hairy cell leukemia (HCL) patients frequently experience opportunistic infections.
  • This suggests a potential impairment in T-cell functioning within HCL patients.
  • Understanding T-cell dynamics is crucial for managing HCL complications.

Purpose of the Study:

  • To investigate T-cell functioning in HCL by analyzing naive and memory T-cell populations.
  • To determine the expression of CD45R0 on CD4+ and CD8+ T cells in HCL patients.
  • To compare T-cell profiles in HCL with healthy subjects and other chronic B-cell leukemias.

Main Methods:

  • Flow cytometry was used to measure CD45R0 expression on CD4+ and CD8+ T cells.
  • Analysis included 23 HCL patients, 13 healthy subjects, and 13 patients with other chronic B-cell leukemias.

Related Experiment Videos

  • T-cell subsets (naive/memory) were quantified based on CD45R0 expression.

    • Main Results:

    • HCL patients with active disease had significantly lower percentages and absolute numbers of CD4+ CD45R0+ T cells compared to healthy controls.
    • No significant differences in CD8+ T cells expressing CD45R0 were observed between HCL patients and controls.
    • Patients with chronic lymphocytic leukemia or leukemic non-Hodgkin's lymphoma showed an elevation in CD45R0-expressing T cells.

    Conclusions:

    • HCL is associated with a reduction in memory CD4+ T cells (CD4+ CD45R0+), suggesting impaired T-cell immunity.
    • The underlying mechanism for reduced CD45R0 expression in HCL CD4+ T cells requires further investigation.
    • These findings contribute to understanding T-cell dysfunction in HCL and its link to infections.