Related Experiment Videos
Identification of an essential glutamate residue in the active site of endoglucanase III from Trichoderma reesei
R Macarron1, J van Beeumen, B Henrissat
1Departamento de Bioquimica, Facultad de Ciencias Biologicas, Universidad Complutense, Madrid, Spain.
FEBS Letters
|January 25, 1993
Abstract:
n-Propyl, n-butyl and n-pentyl beta-cellobiosides with a reactive omega-epoxide in their aglycon completely and irreversibly inactivate endoglucanase III from Trichoderma reesei. The pentyl derivative was found to be most effective. From these affinity labeling experiments evidence was found for the implication of Glu329 in the reaction mechanism. This is discussed in relation to other structural/functional data known for endoglucanase III and several other family A glycanases.