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Related Experiment Videos

Role of somatostatin in the acute immobilization stress-induced GH decrease in rat

A Benyassi1, A Gavaldà, A Armario

  • 1Laboratory of Endocrinological Neurobiology, UA 1197 of CNRS, University of Montpellier, France.

Life Sciences
|January 1, 1993
PubMed
Summary

Immobilization stress rapidly increases somatostatin (SRIF) release from the median eminence (ME) in rats. However, jugular catheterization blunts this SRIF response, suggesting stress-induced changes in growth hormone (GH) regulation.

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Area of Science:

  • Neuroendocrinology
  • Stress Physiology

Background:

  • Stress immobilization (IMO) is known to affect neuroendocrine functions.
  • Somatostatin (SRIF) plays a crucial role in regulating growth hormone (GH) release.
  • The median eminence (ME) is a key site for neurohormone release into the portal system.

Purpose of the Study:

  • To investigate the impact of immobilization stress on somatostatin (SRIF) release from the median eminence (ME) in rats.
  • To examine how jugular catheterization, a procedure associated with stress, influences SRIF release during immobilization stress.
  • To explore the relationship between SRIF release, GH levels, and stress in rats.

Main Methods:

  • Unanesthetized rats were implanted with push-pull cannulas (PPC) in the ME.
  • ME was perfused with artificial cerebrospinal fluid (CSF) under basal, stress (IMO), and recovery conditions.

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  • SRIF levels in perfusates and GH levels in plasma were measured using radioimmunoassay (RIA).
  • A separate group of rats had jugular catheters inserted prior to stress exposure.

    • Main Results:

    • Immobilization stress significantly increased SRIF release from the ME within 15 minutes in rats with only PPC.
    • Rats with both jugular catheters and PPC showed no initial increase in SRIF release during IMO, despite a sharp decrease in plasma GH.
    • While SRIF release eventually increased in the jugular catheter group, the response was delayed and attenuated compared to the control group.
    • Pre-existing differences in food intake, body weight, plasma GH, and basal SRIF release were noted between the two experimental groups.

    Conclusions:

    • Immobilization stress acutely enhances somatostatin (SRIF) release from the median eminence (ME) in rats.
    • Jugular catheterization, potentially due to surgical stress, alters the neuroendocrine response to immobilization, blunting the expected SRIF release.
    • Differences induced by jugular catheter surgery may explain the modified regulation of GH control under acute stress.