Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mosquito DNA polymerase epsilon

S K Lee1, M S Fuchs

  • 1Department of Biological Sciences, University of Notre Dame, IN 46556.

FEBS Letters
|February 1, 1993
PubMed
Summary
This summary is machine-generated.

Researchers purified mosquito DNA polymerase epsilon, a key enzyme for DNA replication. This proliferating cell nuclear antigen (PCNA)-independent enzyme shows intrinsic exonuclease activity and is distinct from DNA polymerase alpha.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recovery of indium from LCD screens of discarded cell phones.

Waste management (New York, N.Y.)·2015
Same author

Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng.

Cancer letters·2001
Same author

A new strategy to predict the neoplastic polyps of the gallbladder based on a scoring system using EUS.

Gastrointestinal endoscopy·2000
Same author

A case of mucin-hypersecreting intrahepatic bile duct tumor associated with pancreatic intraductal papillary mucinous tumor.

Gastrointestinal endoscopy·2000
Same author

The clinical usefulness of ictal SPECT in temporal lobe epilepsy: the lateralization of seizure focus and correlation with EEG.

Epilepsia·2000
Same author

Detection of Helicobacter pylori DNA in human biliary tree and its association with hepatolithiasis.

Digestive diseases and sciences·2000
Same journal

Extending the classical sequence-structure-function paradigm through protein dynamics and context-dependent behavior.

FEBS letters·2026
Same journal

α-Synuclein aggregation landscape from phase separation to neurotoxic intermediates.

FEBS letters·2026
Same journal

Modelling stem cell differentiation related processes-A practical overview for biologists.

FEBS letters·2026
Same journal

Overlapping gut microbiome signatures in aging and disease are characterized by enrichment of medication-associated oral microbes in the gut.

FEBS letters·2026
Same journal

Csk binding to integrin β3 is regulated by tyrosine and threonine phosphorylation of β3.

FEBS letters·2026
Same journal

Mixed-class J-domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly.

FEBS letters·2026
See all related articles

Area of Science:

  • Molecular Biology
  • Enzymology
  • Insect Biochemistry

Background:

  • DNA polymerases are crucial for DNA replication and repair.
  • Mammalian DNA polymerase epsilon plays a vital role in DNA replication.
  • The characterization of insect DNA polymerases is essential for understanding genome replication.

Purpose of the Study:

  • To purify and characterize the mosquito homolog of mammalian DNA polymerase epsilon.
  • To investigate the enzymatic properties and cofactor requirements of mosquito DNA polymerase epsilon.
  • To differentiate mosquito DNA polymerase epsilon from DNA polymerase alpha.

Main Methods:

  • Purification of mosquito DNA polymerase epsilon using hydroxylapatite, single-stranded DNA agarose, and 5' AMP-agarose chromatography.

Related Experiment Videos

  • Assay of polymerase activity using oligo-primed DNA templates.
  • Assessment of stimulation by human and Drosophila proliferating cell nuclear antigen (PCNA).
  • Immunochemical and biochemical analyses to distinguish from DNA polymerase alpha.

    • Main Results:

    • Mosquito DNA polymerase epsilon was successfully purified from larval extracts.
    • The purified enzyme possesses intrinsic 3'-5' exonuclease activity.
    • High polymerase activity was observed with oligo-primed DNA templates.
    • Neither human nor Drosophila PCNA stimulated the enzyme's activity.
    • Evidence confirmed the enzyme's distinction from DNA polymerase alpha.

    Conclusions:

    • The study successfully isolated and characterized mosquito DNA polymerase epsilon.
    • Mosquito DNA polymerase epsilon functions independently of PCNA, similar to its mammalian counterpart.
    • This research provides insights into the diversity and function of DNA polymerases in insects.