Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A PCR strategy that differentiates two alleles for a single base insertion within a T repeat

O Lungu1, S Silverstein

  • 1Department of Microbiology, Columbia University, New York, NY 10032.

Biotechniques
|January 1, 1993
PubMed
Summary

This study introduces a novel PCR method to distinguish the 21-hydroxylase gene from its pseudogene by analyzing thymine (T) repeat numbers. This technique offers an alternative for differentiating gene alleles with varying nucleotide repeat sequences.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Decoding cerebro-spinal signatures of human behavior: Application to motor sequence learning.

NeuroImage·2023
Same author

Dental Professionals' Engagement in Tobacco, Electronic Cigarette, and Cannabis Patient Counseling.

JDR clinical and translational research·2019
Same author

Medical Informatics Training at Yale University School of Medicine.

Yearbook of medical informatics·2016
Same author

Influence of aerobic exercise training on the neural correlates of motor learning in Parkinson's disease individuals.

NeuroImage. Clinical·2016
Same author

Enhancing both motor and cognitive functioning in Parkinson's disease: Aerobic exercise as a rehabilitative intervention.

Brain and cognition·2015
Same author

Neuronal correlates of appetite regulation in patients with schizophrenia: is there a basis for future appetite dysfunction?

European psychiatry : the journal of the Association of European Psychiatrists·2012

Area of Science:

  • Genetics
  • Molecular Biology
  • Biochemistry

Background:

  • Distinguishing functional genes from pseudogenes is crucial for accurate genetic analysis.
  • The 21-hydroxylase gene and its pseudogene share sequence similarities, posing challenges for differentiation.
  • Alleles with varying numbers of repeated nucleotides require precise identification methods.

Purpose of the Study:

  • To develop and validate a new method for differentiating the 21-hydroxylase gene from its pseudogene.
  • To assess the utility of PCR amplification with modified primers and BcgI restriction fragment length polymorphism (RFLP) analysis for this purpose.

Main Methods:

  • Utilized PCR amplification with specifically designed primers.
  • Performed BcgI restriction fragment length polymorphism (RFLP) analysis.

Related Experiment Videos

  • Focused on analyzing the number of thymine (T) repeats in exon 7 of the 21-hydroxylase gene and its pseudogene.

    • Main Results:

    • Successfully differentiated the 7 T-repeat sequence in the 21-hydroxylase gene from the 8 T-repeat sequence in its pseudogene.
    • The combined PCR and BcgI RFLP approach proved effective in distinguishing these alleles.

    Conclusions:

    • The developed PCR and BcgI RFLP method provides a reliable alternative to oligonucleotide hybridization or sequence analysis.
    • This approach is valuable for distinguishing alleles with differing numbers of identical nucleotide repeats, particularly in the 21-hydroxylase gene context.