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Equine T-lymphocyte MHC II expression: variation with age and subset
D P Lunn1, M A Holmes, W P Duffus
1Department of Clinical Veterinary Medicine, University of Cambridge, UK.
Veterinary Immunology and Immunopathology
|January 1, 1993
Summary
Equine Major Histocompatibility Complex (MHC) II is present on T and B lymphocytes. Its expression increases with age, potentially marking memory T-lymphocytes in horses.
Area of Science:
- Immunology
- Equine Immunology
- Leukocyte Antigen Research
Background:
- Monoclonal antibody CVS10 identifies an equine leukocyte antigen.
- This antigen is characterized as equine Major Histocompatibility Complex (MHC) II.
- Equine MHC II is found on T-lymphocytes and B-lymphocytes.
Purpose of the Study:
- To characterize the equine MHC II antigen.
- To investigate the distribution of equine MHC II on lymphocyte subpopulations.
- To examine age-related changes in equine MHC II expression.
Main Methods:
- Characterization of monoclonal antibody CVS10.
- Tissue distribution analysis of the equine antigen.
- Biochemical analysis of the antigen.
- Flow cytometry to assess lymphocyte subpopulations and MHC II expression.
- Age-related expression studies in foals and adult horses.
Main Results:
- Equine MHC II is expressed on T-lymphocytes (CD4+ and CD8+ subpopulations) and B-lymphocytes.
- Neonatal foals exhibit significantly lower lymphocyte MHC II expression compared to adult horses.
- The age-related increase in MHC II expression is primarily observed in the T-lymphocyte subpopulation.
Conclusions:
- Equine MHC II expression on lymphocytes increases with age.
- This age-related change may be linked to the development of memory T-lymphocytes.
- Equine MHC II expression could serve as a marker for memory T-lymphocytes.