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Related Experiment Videos

Changes in synaptosomal glutamate release during postnatal development in the rat hippocampus and cortex

K J Collard1, R Edwards, Y Liu

  • 1Department of Physiology, University of Wales, College of Cardiff, UK.

Brain Research. Developmental Brain Research
|January 15, 1993
PubMed
Summary

Glutamate release from rat brain synaptosomes increases significantly after postnatal day 15. This developmental change in neurotransmitter release may explain why neonatal brains are less vulnerable to ischemic damage.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Neurochemistry

Background:

  • Glutamate is a key excitatory neurotransmitter in the brain.
  • Understanding glutamate release during development is crucial for comprehending brain maturation and vulnerability.
  • Synaptosomes are essential for studying neurotransmitter release mechanisms.

Purpose of the Study:

  • To investigate the developmental changes in potassium-induced glutamate release from rat hippocampal and cortical synaptosomes.
  • To correlate these changes with brain maturation and potential neuroprotection during development.

Main Methods:

  • Utilized radiolabeled [3H]L-glutamate to quantify release.
  • Examined synaptosomes from rats across various developmental stages, from postnatal day 4 (PND 4) to adulthood.

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  • Stimulated neurotransmitter release using potassium (K+) depolarization.

    • Main Results:

    • Synaptosomes from younger rats (PND 4-PND 15) showed significantly lower [3H]L-glutamate release compared to adults.
    • Glutamate release sensitivity increased progressively from PND 15, reaching adult levels.
    • Variations in release sensitivity were observed during early development (PND 4-PND 15).

    Conclusions:

    • The lower glutamate release in neonatal rats may contribute to their relative resistance to ischemic injury.
    • Developmental changes in K+-evoked glutamate release are linked to neural plasticity during critical developmental periods.
    • This study highlights a key neurochemical maturation process influencing brain development and resilience.