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Mapping of multiple intestinal neoplasia (Min) to proximal chromosome 18 of the mouse
1McArdle Laboratory for Cancer Research, University of Wisconsin-Madison 53706.
Abstract:
The Min (multiple intestinal neoplasia) mutation of the mouse has been mapped by analyzing the inheritance of restriction fragment length polymorphisms and simple sequence length polymorphisms in progeny from two intraspecific crosses segregating for the Min mutation. Min, a mutant allele of Apc, the mouse homolog of the human APC (adenomatous polyposis coli) gene, maps to proximal chromosome 18. The synteny between Apc and Mcc, the mouse homolog of the human MCC (mutated in colorectal cancer) gene, is conserved between mouse and human, although the gene order in the Apc to Mcc interval is different from that in the APC to MCC interval.
Insights
The mouse Min mutation, an Apc gene variant, was mapped to chromosome 18 using genetic markers. This finding aids in understanding colorectal cancer gene homologs in mice and humans.
Area of Science:
- Genetics
- Molecular Biology
- Cancer Research
Background:
- The Min mutation in mice is a model for human familial adenomatous polyposis.
- Understanding the genetic location of Min is crucial for studying intestinal neoplasia.
- The mouse Apc gene is homologous to the human APC gene involved in colorectal cancer.
Purpose of the Study:
- To map the genetic location of the Min mutation in mice.
- To analyze the inheritance of genetic markers linked to the Min mutation.
- To compare conserved synteny between mouse and human colorectal cancer gene homologs.
Main Methods:
- Analysis of restriction fragment length polymorphisms (RFLPs).
- Analysis of simple sequence length polymorphisms (SSLPs).
- Segregation analysis in progeny from intraspecific crosses.
Main Results:
- The Min mutation was mapped to proximal chromosome 18 in mice.
- The mouse Apc gene, carrying the Min mutation, is located on chromosome 18.
- Conserved synteny was observed between mouse Apc-Mcc and human APC-MCC gene regions, with differences in gene order.
Conclusions:
- The genetic mapping of the Min mutation provides a valuable tool for further research.
- The study confirms conserved synteny of key cancer-related genes between mice and humans.
- Differences in gene order highlight evolutionary divergence in conserved chromosomal regions.