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Increased nitric oxide synthesis in ulcerative colitis

S J Middleton1, M Shorthouse, J O Hunter

  • 1Department of Gastroenterology, Addenbrooke's Hospital, Cambridge, UK.

Lancet (London, England)
|February 20, 1993
PubMed
Summary
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Increased nitric oxide (NO) production, indicated by higher citrulline levels, was observed in active ulcerative colitis. This suggests NO may play a role in the disease

Area of Science:

  • Gastroenterology
  • Immunology
  • Biochemistry

Background:

  • Nitric oxide (NO) is synthesized by NO synthase, releasing citrulline.
  • Ulcerative colitis (UC) is a chronic inflammatory bowel disease affecting the colon.

Purpose of the Study:

  • To investigate the role of nitric oxide (NO) biosynthesis in active ulcerative colitis (UC).
  • To determine if NO production is elevated in UC tissues.

Main Methods:

  • Measured citrulline concentrations in rectal biopsy specimens from patients with active UC, quiescent UC, and normal histology.
  • Incubated UC biopsy samples with NG-monomethyl-L-arginine (L-NMMA) to assess its effect on citrulline production.

Main Results:

  • Citrulline concentrations were significantly higher in active UC biopsies compared to quiescent UC or normal histology.

Related Experiment Videos

  • Inhibition of NO synthase by L-NMMA reduced citrulline production in UC biopsy samples.

    • Conclusions:

    • Mucosal NO biosynthesis is increased in active ulcerative colitis.
    • Elevated NO production may contribute to the pathogenesis of ulcerative colitis.