Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Modified counterelectrophoresis method for subtyping hepatitis B antigen

M H Hatch

    Journal of Clinical Microbiology
    |September 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Research and the university.

    AAUP bulletin : quarterly publication of the American Association of University Professors·2010
    Same author

    Diabetes care management: a managed care approach.

    WMJ : official publication of the State Medical Society of Wisconsin·1998
    Same author

    Eradication of wild poliovirus from the Americas: wild poliovirus surveillance--laboratory issues.

    The Journal of infectious diseases·1997
    Same author

    Isolation of Echovirus type 11 and Enterovirus type 71 in a day care winter outbreak.

    The Journal of the Arkansas Medical Society·1987
    Same author

    Hemolytic-uremic syndrome--an outbreak in Sacramento, California.

    The Western journal of medicine·1986
    Same author

    Acute hemorrhagic conjunctivitis caused by enterovirus type 70: an epidemic in American Samoa.

    The American journal of tropical medicine and hygiene·1985
    Same journal

    Ebola laboratory preparedness at frontline hospitals: can we or can't we?

    Journal of clinical microbiology·2026
    Same journal

    Reporting macrolide-resistant <i>Mycoplasma pneumoniae</i>: a diagnostic obligation?

    Journal of clinical microbiology·2026
    Same journal

    Diagnostic value of HHV-6A/B genotyping in immunocompromised adults.

    Journal of clinical microbiology·2026
    Same journal

    Multicenter performance evaluation of the Simplexa <i>C. auris</i> Direct assay for the detection of <i>Candida auris</i> colonization in bilateral axilla/groin swabs.

    Journal of clinical microbiology·2026
    Same journal

    Comparison of blood culture contamination rates with standard practice versus two blood diversion devices at a single institution.

    Journal of clinical microbiology·2026
    Same journal

    Risk assessment and mitigation of hepatitis C virus RNA carryover contamination in a reflex testing algorithm.

    Journal of clinical microbiology·2026
    See all related articles

    A new counterelectrophoresis (CEP) method enhances hepatitis B surface antigen (HBSAg) subtyping sensitivity. This improved technique successfully identified subtypes in samples previously undetectable by standard methods.

    Area of Science:

    • Hepatology
    • Immunology
    • Virology

    Background:

    • Hepatitis B virus (HBV) infection is a global health concern.
    • Accurate subtyping of hepatitis B surface antigen (HBSAg) is crucial for epidemiological studies and vaccine development.
    • Existing methods like immunodiffusion (ID) have limitations in sensitivity for HBSAg subtyping.

    Purpose of the Study:

    • To develop and validate a modified counterelectrophoresis (CEP) method for sensitive HBSAg d and y subtyping.
    • To compare the sensitivity of the modified CEP method against the conventional immunodiffusion (ID) method.

    Main Methods:

    • A modified counterelectrophoresis (CEP) technique was developed.
    • HBSAg of known subtype was pre-diffused into agarose gel to absorb common antibodies from subtyping antiserum.

    Related Experiment Videos

  • Electrophoresis was performed to allow specific antigen-antibody reactions for d and y subtype determination.

    • Main Results:

    • The modified CEP method demonstrated significantly higher sensitivity compared to the ID method.
    • Sixty-two sera unsubtypeable by ID were successfully subtyped using the modified CEP method.
    • Sera unsubtypeable by ID had significantly lower HBSAg complement fixation titers, suggesting lower antigen levels.

    Conclusions:

    • The modified CEP method offers a more sensitive approach for HBSAg d/y subtyping.
    • This enhanced sensitivity is particularly valuable for analyzing samples with low HBSAg levels.
    • Further advancements in subtyping techniques may be necessary for certain HBSAg-positive sera.