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Related Experiment Videos

P-glycoprotein expression in normal and reactive bone marrows

S Hegewisch-Becker1, M Fliegner, T Tsuruo

  • 1Medical University Clinic of Hamburg, Department of Oncology and Haematology, Germany.

British Journal of Cancer
|March 1, 1993
PubMed
Summary
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P-glycoprotein (P-gp) expression was found in 51% of normal and reactive bone marrow samples. This P-gp expression may occur in CD-34 negative cells, suggesting potential reinduction under specific conditions.

Area of Science:

  • Hematology
  • Molecular Biology
  • Immunocytochemistry

Background:

  • P-glycoprotein (P-gp), encoded by the mdr1 gene, is a transmembrane efflux pump.
  • P-gp expression is typically associated with multidrug resistance and has been primarily observed in CD-34 positive bone marrow cells.

Purpose of the Study:

  • To investigate P-glycoprotein (P-gp) expression in normal and reactive bone marrow.
  • To determine if P-gp expression occurs in CD-34 negative bone marrow cells.

Main Methods:

  • Immunocytochemistry using monoclonal antibodies (mAb C219, mAb MRK16).
  • Alkaline phosphatase anti-alkaline phosphatase method.
  • Polymerase chain reaction (PCR) for mdr1 gene expression in a subset of samples.
  • Staining for CD-34 surface marker.

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Main Results:

  • P-gp expression was detected in 27 out of 53 (51%) bone marrow samples.
  • Positive P-gp expression ranged from 2% to 80% (mean 24%).
  • Using a 10% cutoff, 22% of normal and 46% of reactive bone marrows were positive for P-gp.
  • No significant correlation was found between P-gp expression, diagnosis, or age.
  • CD-34 positivity was less than 1% in tested samples, suggesting P-gp expression may occur in CD-34 negative cells.

Conclusions:

  • P-glycoprotein (P-gp) is expressed in a significant proportion of non-malignant, non-chemotherapy-exposed bone marrows.
  • The study suggests that P-gp expression can be reinduced in CD-34 negative bone marrow cells under certain conditions.
  • Further research is needed to elucidate the mechanisms and implications of P-gp reinduction in CD-34 negative cells.