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Host reaction against alginate-polylysine microcapsules containing living cells
G M Vandenbossche1, M E Bracke, C A Cuvelier
1Laboratory of Pharmaceutical Technology, University of Ghent, Belgium.
The Journal of Pharmacy and Pharmacology
|February 1, 1993
Summary
Researchers evaluated host reactions to intraperitoneal implants in mice. Encapsulated tumor cells triggered a stronger immune response compared to empty microcapsules or non-tumorigenic cells, indicating microcapsule implants can be assessed via peritoneal lavage and capsule histology.
Area of Science:
- Immunology
- Biomaterials Science
- Cancer Research
Background:
- Assessing host responses to implanted biomaterials is crucial for understanding biocompatibility.
- Microcapsules are utilized for drug delivery and tissue engineering, necessitating evaluation of their interaction with the host immune system.
- Tumorigenic cells can elicit distinct host reactions compared to non-tumorigenic cells.
Purpose of the Study:
- To investigate the cellular host reaction to intraperitoneal microcapsule implants in syngeneic and nude mice.
- To compare the host response to empty microcapsules, encapsulated non-tumorigenic cells, and encapsulated tumorigenic cells.
- To establish methods for evaluating host reactions to intraperitoneal implants.
Main Methods:
- Intraperitoneal injection of saline, empty microcapsules, aggregates of MO4 cells, encapsulated non-tumourogenic MO cells, and encapsulated MO4 cells into mice.
- Evaluation of host reaction 4 days post-injection by counting peritoneal leucocytes.
- Histological examination of recovered microcapsules to assess adherent cells.
Main Results:
- Mice injected with empty microcapsules or encapsulated non-tumorigenic MO cells showed significantly fewer peritoneal leucocytes compared to those with encapsulated tumorigenic MO4 cells.
- Histological analysis revealed a significantly higher number of cells adhering to microcapsules containing cells versus empty microcapsules.
- These findings indicate a differential host response based on the presence and tumorigenicity of encapsulated cells.
Conclusions:
- The cellular host reaction to intraperitoneal implants can be effectively evaluated using peritoneal leucocyte counts.
- Histology of recovered microcapsules provides valuable insights into cellular adherence and host response.
- Microcapsule encapsulation influences the host's immune reaction, with tumorigenic cells eliciting a more pronounced response.