Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

STE6, the yeast a-factor transporter

S Michaelis1

  • 1Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

Seminars in Cell Biology
|February 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Label-free analysis of GPCR-stimulation: The critical impact of cell adhesion.

Pharmacological research·2016
Same author

Label-free monitoring of cell-based assays: combining impedance analysis with SPR for multiparametric cell profiling.

Biosensors & bioelectronics·2013
Same author

[PPI-sensitive diarrhea - unusual case of an adolescent with sporadic gastrinoma].

Deutsche medizinische Wochenschrift (1946)·2012
Same author

Combination of bexarotene and psoralen-UVA therapy in a patient with Mycosis fungoides.

Dermatology (Basel, Switzerland)·2004
Same author

Subcutaneous infection with Mycobacterium abscessus in a renal transplant recipient.

Dermatology (Basel, Switzerland)·2004
Same author

Evaluation of living conditions of free-ranging animals by automated chronobiological analysis of behavior.

Behavior research methods, instruments, & computers : a journal of the Psychonomic Society, Inc·2003

Saccharomyces cerevisiae uses a non-classical pathway for alpha-factor secretion, mediated by the STE6 transporter. This study dissects the substrate-transporter interactions of this key ATP-binding cassette (ABC) protein.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Most eukaryotic proteins use the classical ER-Golgi pathway for secretion.
  • The yeast alpha-factor pheromone is an exception, utilizing a non-classical secretion mechanism.

Purpose of the Study:

  • To investigate the non-classical secretion of Saccharomyces cerevisiae alpha-factor.
  • To analyze the function of STE6, an ATP-binding cassette (ABC) transporter, in alpha-factor export.

Main Methods:

  • Utilizing yeast genetics for molecular manipulations.
  • Probing substrate-transporter interactions through genetic analysis.

Main Results:

  • Identified STE6 as the transporter mediating alpha-factor secretion.

Related Experiment Videos

  • Demonstrated that STE6 is an ABC protein with a known physiological substrate.

    • Conclusions:

    • STE6 plays a crucial role in the non-classical secretion of alpha-factor.
    • Yeast provides a model system to study ABC protein function and substrate interactions.